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目的通过采用高效液相法测定河南不同产地夏枯草中熊果酸、齐墩果酸的含量来比较夏枯草质量的优劣,为夏枯草的质量控制方法提供一定的依据。方法测定熊果酸、齐墩果酸色谱条件为Betasil C18(250 mm×4.6 mm,5μm);流动相:磷酸二氢钠缓冲液[取磷酸二氢钠7.8 g加水至1 000 mL]-甲醇(14∶86);流速:0.8 mL.min-1;柱温:25℃;检测波长:210 nm;结果熊果酸在0.554~5.34μg内线性关系良好,r=0.999 7,平均回收率为97.45%,RSD为0.92%。齐墩果酸在0.28~2.24μg内线性关系良好,r=0.999 4,平均回收率为97.61%,RSD为0.87%。结论该方法简单、准确并且专属性强。
Objective To determine the quality of Prunella vulgaris by high performance liquid chromatography (HPLC) method for the determination of ursolic acid and oleanolic acid in Prunella vulgaris from different regions of Henan Province, and to provide a basis for the quality control of Prunella vulgaris. Methods The chromatographic conditions of ursolic acid and oleanolic acid were Betasil C18 (250 mm×4.6 mm, 5 μm). The mobile phase was sodium dihydrogen phosphate buffer (sodium dihydrogen phosphate, 7.8 g, water was added to 1 000 mL). (14:86);Flow rate:0.8 mL.min-1;Column temperature:25°C; Detection wavelength:210 nm;Results The ursolic acid has good linearity in 0.554~5.34μg,r=0.999 7,The average recovery rate 97.45%, RSD 0.92%. The linear relationship of oleanolic acid was good in 0.28~2.24μg, r=0.999 4, the average recovery was 97.61%, and the RSD was 0.87%. Conclusion This method is simple, accurate and specific.