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目的：了解黄芪多糖对体外培养成骨细胞增殖、分化能力的影响。方法：采用水煮醇沉法提取黄芪多糖，用ＴＬＣ进行定性，用比色法定量，用含高低两种剂量（０．５ｍ ｇ／ｍ ｌ，５．０ｍ ｇ／ｍ ｌ）的黄芪多糖的培养液体外培养成骨细胞，ＭＴＴ法及ＡＬＰ比活性测定观察成骨细胞增殖率及细胞活性变化。结果：低浓度黄芪多糖（０．５ｍ ｇ／ｍ ｌ）及高浓度黄芪多糖（５．０ｍ ｇ／ｍ ｌ）短期时（２ 天）促进成骨细胞增殖；高浓度（５．０ｍ ｇ／ｍ ｌ）长期（４ 天）抑制成骨细胞增殖，降低其活性。结论：黄芪多糖对成骨细胞增殖、活性有双向调节作用
Objective: To investigate the effects of astragalus polysaccharides on the proliferation and differentiation of osteoblasts in vitro. Methods: Astragalus polysaccharides were extracted by boiled alcohol precipitation and qualitative by TLC. Quantitative method was used to quantify the content of astragalus polysaccharides at both high and low doses (0.5m g/ml, 5.0m g/ml). Cultured osteoblasts were cultured in vitro. MTT assay and ALP specific activity assay were used to observe the osteoblast proliferation rate and cell activity changes. RESULTS: Low concentrations of astragalus polysaccharide (0.5 mg/ml) and high concentration of astragalus polysaccharide (5.0 mg/ml) promoted osteoblast proliferation in short-term (2 days); high concentrations (5.0 m g/m) l) Prolonged (4 days) inhibition of osteoblast proliferation and reduced its activity. Conclusion: Astragalus polysaccharide has two-way adjustment effect on the proliferation and activity of osteoblasts