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为了探明吲哚丁酸(Indole-3-butyric acid,IBA)对辣椒(Capsicum annuum L.)下胚轴不定根发生的诱导机制,采用IBA浸蘸后扦插的方法,研究了外源IBA处理后辣椒下胚轴不定根发生进程、糖类物质转化与不定根发生相关基因表达情况。结果表明:外源IBA诱导辣椒下胚轴不定根发生具有浓度依存效应,150μmol·L~(-1) IBA处理后不定根发生区段长度、不定根数及平均根长分别是对照的3.47倍、3.28倍和1.91倍;150μmol·L~(-1) IBA上调了不定根启动相关基因Ca ARL1和Ca Cyc B1的表达,下胚轴的不定根原基发生区域明显增加;此外,150μmol·L~(-1) IBA还上调了下胚轴不定根发生区段糖代谢相关基因Ca CWI和Ca STP的表达,可溶性糖、淀粉含量及细胞壁转化酶活性显著提高。结果表明适宜浓度IBA处理可以促进辣椒下胚轴不定根发生区段糖类物质的积累和转化,诱导辣椒下胚轴不定根的发生。
In order to investigate the induction mechanism of adventitious root formation in hypocotyls of pepper (Capsicum annuum L.) by Indole-3-butyric acid (IBA), IBA was used to study the induction of adventitious root formation. The process of adventitious root formation in the hypocotyls of pepper and the expression of genes related to adventitious root transformation and carbohydrate transformation. The results showed that exogenous IBA induces adventitious root formation of pepper hypocotyls in a concentration-dependent manner. The length of adventitious roots, the number of adventitious roots and the average root length after treatment with 150μmol·L -1 IBA are 3.47 and 3.28 times And 1.91 folds, respectively. 150μmol·L -1 IBA up-regulated the expression of Ca ARL1 and Ca Cyc B1 in adventitious roots, and the area of adventitious root primordium in hypocotyls significantly increased. In addition, 150μmol·L -1 IBA up- IBA also up-regulated the expression of Ca CWI and Ca STP related to sugar metabolism in adventitious roots of hypocotyls, and the soluble sugar, starch content and cell wall invertase activity were significantly increased. The results showed that the appropriate concentration of IBA could promote the accumulation and transformation of sugarcane in the adventitious root of the hypocotyls and induce the adventitious root formation in the hypocotyls of pepper.