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目的：研究经逆转录病毒载体的介导将脑源性神经营养因子（ＢＤＮＦ）ｃＤＮＡ导入大鼠成肌细胞，为体内移植治疗打下基础。方法：采用电穿孔法将重组大鼠ＢＤＮＦｃＤＮＡ导入ＰＡ３１７包装细胞，获取高滴度的病毒上清转染成肌细胞。Ｇ４１８筛选转染的成肌细胞，采用Ｎｏｒｔｈｅｍ杂交和ＰＣ１２细胞存活检测。结果：证实转染细胞内有ＢＤＮＦｍＲＮＡ的表达并具有促进细胞存活的生物学活性。结论：ＢＤＮＦｃＤＮＡ可在真核细胞内表达并具有生物学效应，可用于神经系统基因治疗的研究。
OBJECTIVE: To study the introduction of BDNF cDNA into rat myoblasts mediated by retroviral vector and lay the foundation for transplantation in vivo. METHODS: Recombinant rat BDNF cDNA was introduced into PA317 packaging cells by electroporation, and the high titer virus supernatant was obtained and transfected into myoblasts. Transfected myoblasts were screened by G418 using Northem hybridization and PC12 cell survival assays. Results: The expression of BDNF mRNA was confirmed in the transfected cells and had the biological activity of promoting cell survival. Conclusion: BDNF cDNA can be expressed in eukaryotic cells and has biological effects. It can be used in gene therapy of nervous system.