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目的:建立以HPLC法测定银密片中腺苷含量的方法。方法:采用Shim-pack VP-ODS C_(18)(250 mm×4.6 mm,5μm)柱,以乙腈:0.04 mol·L~(-1)磷酸二氢钾溶液(7:93)为流动相,检测波长:260nm,流速1.0ml·min~(-1)。结果:腺苷在21.32～170.56 ng范围内线性关系良好,r=0.999 9,平均加样回收率为99.4%,RSD为1.6%。结论:本方法操作简便,方法可靠,结果准确、灵敏度高,重复性好,可作为该产品质量控制的方法。
Objective: To establish a HPLC method for the determination of adenosine in silver secret tablets. Methods: The mobile phase consisted of Shim-pack VP-ODS C_ (18) (250 mm × 4.6 mm, 5 μm) with acetonitrile: 0.04 mol·L -1 potassium dihydrogen phosphate solution (7:93) Detection wavelength: 260nm, flow rate 1.0ml · min ~ (-1). Results: The linearity of adenosine in the range of 21.32-170.56 ng was good, r = 0.999 9, the average recovery was 99.4% and the RSD was 1.6%. Conclusion: The method is simple and convenient, the method is reliable, the result is accurate, the sensitivity is high and the repeatability is good. It can be used as the method of product quality control.