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目的:通过对口腔扁平苔藓(OLP)局部组织及外周血中IL-2、IL-10表达进行研究,进一步探讨OLP发病机制。方法:应用免疫组织化学SABC法检测30例OLP中IL-2、IL-10的表达数量及分布,同时用ELISA法检测外周血中IL-2、IL-10的含量。结果:①在病损组织中,OLP组中IL-2、IL-10表达显著高于对照组(P<0.05)。IL-2主要分布在淋巴细胞浸润带,在基底膜附近较集中;IL-10主要分布在固有层,远离基底膜。②在外周血中,糜烂型OLP组IL-2、IL-10含量明显高于对照组和非糜烂型OLP组(P<0.05)。结论:①根据IL-2、IL-10在OLP中表达和分布情况,提示IL-2、IL-10在OLP发病机制中参与了机体免疫的调节和介导作用。②外周血中IL-10高表达,提示糜烂型OLP患者全身的免疫反应参与了本病的形成或可能伴有全身免疫系统功能紊乱。
OBJECTIVE: To investigate the pathogenesis of OLP by studying the expression of IL-2 and IL-10 in local tissues and peripheral blood of oral lichen planus (OLP). Methods: Immunohistochemical SABC method was used to detect the expression and distribution of IL-2 and IL-10 in 30 cases of OLP. Meanwhile, the levels of IL-2 and IL-10 in peripheral blood were measured by ELISA. Results: ①In diseased tissue, the expression of IL-2 and IL-10 in OLP group was significantly higher than that in control group (P <0.05). IL-2 mainly distributed in the lymphocyte infiltration zone, more concentrated in the vicinity of the basement membrane; IL-10 mainly in the lamina propria, away from the basement membrane. ② In peripheral blood, the content of IL-2 and IL-10 in erosive OLP group was significantly higher than that in control group and non-erosive OLP group (P <0.05). Conclusion: ① According to the expression and distribution of IL-2 and IL-10 in OLP, IL-2 and IL-10 are involved in the regulation and mediation of immune system in the pathogenesis of OLP. ② high expression of IL-10 in peripheral blood, suggesting that the systemic immune response in patients with erosive OLP is involved in the formation of the disease or may be associated with systemic immune system dysfunction.