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【目的】建立石淋通片高效液相色谱(HPLC)法指纹图谱研究方法,并采用广金钱草指纹图谱评价石淋通片的质量。【方法】采用HPLC法分析,色谱柱为Zorbax SB-Aq,流动相为乙腈—体积分数0.05%磷酸溶液(梯度洗脱),检测波长为270 nm,流速为1 mL/min,柱温为40℃。【结果】不同厂家石淋通片均具有广金钱草的11个黄酮类共有峰,但不同厂家产品质量有很大差异。【结论】采用指纹图谱与含量测定相结合可作为石淋通片质量控制方法之一。
【OBJECTIVE】 To establish a method for the determination of Shilin tablet by high performance liquid chromatography (HPLC) fingerprinting method. 【Method】 The chromatographic column was Zorbax SB-Aq. The mobile phase was acetonitrile-0.05% phosphoric acid solution (gradient elution). The detection wavelength was 270 nm, the flow rate was 1 mL / min and the column temperature was 40 ℃. 【Result】 There were 11 total flavones shared among different plants of Shilin tablet, but there were great differences among different manufacturers. 【Conclusion】 The combination of fingerprint and content determination can be used as one of the quality control methods of Shilin tablet.