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从患有溃烂病的皱纹盘鲍幼鲍体上,分离到一种致病性细菌。经人工感染试验,证实该菌为皱纹盘鲍幼鲍溃烂病的病原菌。显微镜观察该细菌为革兰氏阴性,极生1~3根鞭毛,能运动的杆菌,大小为0.7~0.8×2.3~2.8um。经细菌分类试验鉴定,该菌生长最适温度为20~30℃,最适pH值5.5~8.5。该菌在培养基B上产生蓝色的荧光素。能利用葡萄糖产酸不产气,果糖、D-半乳糖、甘露糖、木糖、阿拉伯糖、海藻糖发酵反应阳性,不能发酵乳糖、麦芽糖、鼠李糖、蔗糖,可分解山梨醇、肌醇、肌苷。过氧化氢酶、脲酶、氧化酶、及明胶液化反应阳性;能利用柠檬酸,不还原亚硝酸盐,不产生吲哚和硫化氢,MR和V.P.试验阴性。初步鉴定该病原菌为荧光假单胞杆菌。还进行了病原菌药敏试验,该菌对卡那霉素、呋喃唑酮、恶喹酸敏感,这些药物可作为防治该病的首选药。
A pathogenic bacterium is isolated from abalone abalone and juvenile abalone. The artificial infection test, confirmed that the bacteria for the abalone abalone Bao abalone pathogenic bacteria. Microscopic observation of the bacteria is Gram-negative, polar 1 to 3 flagella, can move bacteria, the size of 0.7 ~ 0.8 × 2.3 ~ 2.8um. The bacterial classification test identified the optimum temperature for the growth of the bacteria 20 ~ 30 ℃, the optimum pH value of 5.5 to 8.5. The bacterium produces blue fluorescein on medium B. Can produce glucose by using glucose, fructose, D-galactose, mannose, xylose, arabinose, trehalose fermentation reaction was positive, can not ferment lactose, maltose, rhamnose, sucrose, degradable sorbitol, inositol , Inosine. Catalase, urease, oxidase and gelatin liquefaction positive; can use citric acid, does not reduce nitrite, does not produce indole and hydrogen sulfide, MR and V. P. Test negative. Preliminary identification of the pathogen is Pseudomonas fluorescens. Also conducted drug susceptibility testing of pathogens, the bacteria kanamycin, furazolidone, oxolinic acid sensitive, these drugs can be used as the drug of choice for the prevention and treatment of the disease.