肿瘤坏死因子(TNF)在体内具有抗利什曼原虫的作用,但其本身却不能直接杀灭利什曼原虫。本文观察了体外TNF激活巨噬细胞(Mφ)后杀灭原虫的能力。rHuTNF(基因重组人肿瘤坏死因子)及纯化天然MuTNF(纯化天然鼠肿瘤坏死因子)激活Mφ后杀灭胞内大型利什曼原虫(L.major)。Mφ铺于载玻片上,置于37℃24h后,再加rHuTNF或MuTNF,于37℃放置24h,再按原虫:Mφ=10:1的比例加L-major,原虫,34℃过夜,染色镜检计数。结果证明当用100U/ml的rHuTNF激活Mφ后, Tumor necrosis factor (TNF) has an anti-Leishmania effect in the body, but itself can not kill Leishmania directly. In this paper, the ability of killing protozoa after TNF activated macrophages (Mφ) in vitro was observed. rHuTNF (recombinant human tumor necrosis factor) and purified native MuTNF (purified native murine tumor necrosis factor) kill M. intracellularis L. major after activating Mφ. Mφ were plated on glass slides and incubated at 37 ℃ for 24 h. After addition of rHuTNF or MuTNF, the plate was incubated at 37 ℃ for 24 h. Then L-major protozoal was added at a ratio of Mφ = 10: 1, Check the number. The results demonstrated that when Mφ was activated with 100 U / ml of rHuTNF,