由Hamburger和Salmon所创建的人肿瘤克隆形成分析(HTCA)是确定新分离的肿瘤细胞化学敏感性的最佳方法之一。回顾性研究表明,体外对某一药物的敏感性与带瘤患者的临床反应是非常吻合的。然而,这一方法也有一些局限性,包括不容易获得真正单一细胞悬液,对多种类型的肿瘤来说克隆形成率低。同一类型甚至相同组织学类型的肿瘤其成克 The Human Tumor Cloning Assay (HTCA), created by Hamburger and Salmon, is one of the best ways to determine the chemical sensitivity of newly isolated tumor cells. Retrospective studies have shown that in vitro sensitivity to a drug is very consistent with the clinical response of patients with tumors. However, this method also has some limitations, including that it is not easy to obtain a truly single cell suspension, and the cloning rate is low for many types of tumors. Tumors of the same type or even the same histological type of tumor