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作者用多聚酶链反应(PCR)技术以已知人类免疫缺陷病毒(HIV)阳性感染者的PBMC样品检测HIV感染到卡波济肉瘤(KS)出现这一段临床无症状期的人疱疹病毒8型(HHV-8)DNA序列。 HIV阳性患者为6例KS患者及其配偶。他们分别于1985～1989年被发现感染HIV,于1994～1995年出现KS。其中5例白人由同性性接触感染,第6例黑人为异性性接触感染,感染时平均年龄为31岁(24～41岁)。在治疗过程中每年进行随访观察,采集血清和淋巴细胞样品检测CD4细胞计数、P24抗原、抗P24抗体、HIV-1RNA病毒复制(VC)量以及HHV-8 DNA序列各项生物
The authors used polymerase chain reaction (PCR) technology to detect human immunodeficiency virus type 8 (HSV) in HIV-infected Kaposi’s sarcoma (KS) from a PBMC sample of a known human immunodeficiency virus (HIV) HHV-8) DNA sequence. HIV-positive patients were 6 KS patients and their spouses. They were found to have been infected with HIV from 1985 to 1989, and KS from 1994 to 1995. Five of the whites were infected by same-sex contacts, and the 6th black was heterosexually infected with an average age of 31 years (24-41 years) when infected. The follow-up observation was conducted every year during treatment. Serum and lymphocyte samples were collected to detect CD4 cell count, P24 antigen, anti-P24 antibody, HIV-1 RNA viral replication (VC) and HHV-8 DNA sequences