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目的:为扩大和提高甘肃道地药材秦艽资源的利用效率。方法:以秦艽种子的萌发所获得的幼芽作为外植体,诱导形成愈伤组织;筛选诱导愈伤组织产生的不同培养基和培养条件,并继代培养;应用HPLC法测定龙胆苦苷的含量。结果:秦艽愈伤组织生长的最佳培养基为MS+2,4-D(1.0 mg/L)+6-BA(0.5 mg/L),最佳继代培养基为MS+2,4-D(1.0 mg/L)+6-BA(0.5 mg/L)+NAA(0.5 mg/L),最佳继代时间为30 d。龙胆苦苷积累量在指数生长后期(28～30 d)达最高,为0.78 mg/g DW。结论:筛选出了适宜的秦艽愈伤组织培养方法。秦艽愈伤组织在固体培养基上的生长曲线呈“S”型,且愈伤组织中龙胆苦苷的积累量随愈伤组织增殖而不断增加。
Objective: To expand and improve the utilization efficiency of Gentiana macrophylla resources in Gansu Province. Methods: The shoots obtained from germination of Gentiana macrophylla seedlings were used as explants to induce the formation of callus. The different culture media and culture conditions of callus induction were selected and subcultured. The content of gentiopicroside Content. Results: The best medium for callus growth was MS + 2,4-D (1.0 mg / L) + 6-BA (0.5 mg / L) D (1.0 mg / L) + 6-BA (0.5 mg / L) + NAA (0.5 mg / L) The accumulation of gentiopicroside reached the highest at 0.78 mg / g DW in the late exponential growth period (28-30 days). Conclusion: The suitable method of callus culture of Gentiana macrophylla was screened out. The growth curve of Gentiana macrophylla callus was “S” in solid medium, and the accumulation of gentiopicroside in callus increased with callus proliferation.