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目的 探讨阿霉素肾病幼年大鼠肾损伤早期 ,肾组织血管紧张素Ⅱ 1型受体 (AT1)与核转录因子 (NF)κB(P65/Rel A)表达的趋势、相关性及其潜在的病理意义 ,及给予血管紧张素转换酶抑制剂苯那普利和血管紧张素受体阻断剂氯沙坦的干预影响。方法 以阿霉素肾病幼年大鼠为实验性肾病模型 ,于肾病早期第 1、2、3周观察大鼠蛋白尿、血生化各指标的变化。用免疫组化方法检测大鼠肾组织AT1蛋白表达 ,用原位杂交方法检测大鼠肾组织P65/Rel AmRNA表达的情况 ,并从时相和组织定位表达的趋势上评价AT1和P65/Rel A表达的相关性。结果 ( 1)阿霉素注射后第 1周大鼠即出现明显蛋白尿 ,于第 3周即达大量蛋白尿程度 ( 12 3 2± 7 7)mg/ 2 4h ,同时血生化各指标趋于增高。肾小管中可见大量蛋白管型 ,肾间质中可见大量炎性细胞浸润。 ( 2 )肾小管间质区AT1蛋白和P65/Rel AmRNA表达趋势明显上调 ,第 1、2、3周AT1组化半定量分别为 ( 19 8± 1 1) %、( 2 5 0±2 6 ) %、( 37 1± 1 0 ) % ,假手术组 :( 10 3± 0 8) %、( 10 4± 1 6 ) %、( 10 2± 1 5 ) % ,AT1蛋白主要分布于各级肾小管上皮细胞胞浆和核膜 ,P65/Rel AmRNA表达于小管上皮细胞胞浆 ,随病变的进展 ,其核转位趋势明显增加 ,阳染信号由胞浆转至细胞核 ,P65/R
Objective To investigate the expression of angiotensin Ⅱ type 1 receptor (AT1) and nuclear factor kappa B (P65 / Rel A) in kidneys of adriamycin-induced nephropathy rats at early stage of renal injury. Pathological significance, and administration of angiotensin converting enzyme inhibitor benazepril and angiotensin receptor blockers losartan intervention. Methods Adriamycin nephropathy in young rats as experimental nephropathy model, in the early 1, 2, 3 weeks of nephropathy observed proteinuria, blood biochemical changes in various indicators. Immunohistochemistry was used to detect the expression of AT1 protein in renal tissues of rats. The expression of P65 / Rel AmRNA in rat renal tissues was detected by in situ hybridization and the expression of AT1 and P65 / Rel A Correlation of expression. Results (1) Significant albuminuria occurred in the first week after injection of doxorubicin, reaching a significant level of proteinuria (12 3 2 ± 7 7) mg / 24 h at the third week, and the indexes of blood biochemistry tended to Increase. A large number of renal tubular protein tube can be seen in the renal interstitium can be seen in a large number of inflammatory cell infiltration. (2) The expressions of AT1 protein and P65 / Rel AmRNA in the tubulointerstitial region were significantly up-regulated. The AT1 histochemical semiquantitative levels in the first and second week were (19 8 ± 1 1)%, (2 50 ± 2 6) ), (37 1 ± 10)%, sham operation group (10 3 ± 0 8)%, (10 4 ± 1 6)% and (10 2 ± 1 5)% respectively. P65 / RelAmRNA was expressed in the cytoplasm of tubular epithelial cells in the cytoplasm and nuclear membrane of tubular epithelial cells. With the progression of the lesion, the nuclear translocation tendency of P65 / RelAmRNA increased obviously.