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目的探讨肺耐药相关蛋白(LRP)的表达与儿童白血病的关系及临床意义。方法应用逆转录-聚合酶链反应法(RT-PCR)测定52例不同类型儿童急性白血病及6名正常对照儿童的基因表达。结果(1)23例初治儿童急性白血病患者中9例LRP基因表达阳性。6例正常对照LRP基因表达阴性。LRP基因表达阳性者完全缓解率低于表达阴性者。(2)52例儿童急性白血病患儿,32例ALL,LRP阳性8例;20例ANLL,LRP阳性17例。(3)52例儿童急性白血病患儿,初治组23例,LRP阳性9例;初次复发组16例,阳性7例;多次复发组13例,阳性9例。结论LRP基因存在原发耐药,LRP是影响儿童白血病化疗结果及预后的重要因素之一。儿童急性白血病患儿ANLL相对于ALL缓解率低、预后差可能与LRP的表达有关。LRP的表达随着白血病患儿多次复发呈上升过程。
Objective To investigate the relationship between the expression of lung resistance-related protein (LRP) and childhood leukemia and its clinical significance. Methods RT-PCR was used to detect the gene expression in 52 children with acute leukemia and 6 normal controls. Results (1) The expression of LRP gene in 9 cases of 23 newly diagnosed children with acute leukemia was positive. Six cases of normal control LRP gene expression was negative. The complete remission rate of LRP gene positive patients was lower than that of negative ones. (2) 52 cases of children with acute leukemia, 32 cases of ALL, LRP positive in 8 cases; 20 cases of ANLL, LRP positive in 17 cases. (3) Among 52 children with acute leukemia, 23 cases were initially treated and LRP was positive in 9 cases. The initial recurrent group was 16 cases and the positive was 7 cases. The multiple recurrent group was 13 cases and the positive was 9 cases. Conclusion LRP gene has primary drug resistance. LRP is one of the important factors affecting the outcome and prognosis of childhood leukemia chemotherapy. Children with acute leukemia ANLL relative to ALL low remission rate, poor prognosis may be related to the expression of LRP. LRP expression with leukemia in children with multiple recurrence was up.