论文部分内容阅读
根据烟草环斑病毒 (TRSV)外壳蛋白基因非编序列设计的引物P1,P2 ,用感病及健康组织总RNA为模板 ,进行cDNA合成和PCR试验 ,感病组织中扩增出了 6 0 0bp的目的片段 ,而健康组织中无此扩增带。摸索各项实验条件 ,建立了RT -PCR检测烟草环斑病毒 (TRSV)的方法。
According to the primers P1 and P2 designed by the non-coding sequence of coat protein gene of tobacco ring spot virus (TRSV), cDNA synthesis and PCR test were carried out by using the total RNA of susceptible and healthy tissues as a template. Six hundred bp Of the purpose of the fragment, but healthy tissue without amplification bands. Explore the experimental conditions, the establishment of RT-PCR detection of tobacco ring spot virus (TRSV) method.