论文部分内容阅读
对44例喉鳞癌和16例声带息肉患者采用原位核酸杂交技术探测其人乳头瘤病毒（HPV）6B、11、16、18型DNA同源序列及LSAB免疫组化法探测其p53蛋白的表达。结果：①喉癌与HPV16／18杂交阳性率为43．2％（19／44），声带息肉为12．5％（2／16）（P＜0．05）；②喉癌p53蛋白阳性率为56．8％（25／44），声带息肉全部为阴性；③喉癌HPV16／18杂交及p53蛋白均呈阳性反应者12／44，占HPV16／18杂交阳性标本的63．2％（12／19），占p53蛋白阳性反应标本的48．0％（12／25）。表明：HPV16／18型感染和p53蛋白表达与喉癌的发生有密切关系，并且二者可能有协同作用。
44 cases of laryngeal squamous cell carcinoma and 16 cases of vocal cord polyp were detected by in situ hybridization in situ hybridization to detect HPV type 6B, 11, 16 and 18 DNA and LSAB immunohistochemistry to detect the expression of p53 protein expression. Results: ① The positive rate of laryngeal carcinoma was HPV16 / 18 hybridization (43.2%, 19/44), vocal cord polyp was 12.5% (2/16) (P <0.05); ② The positive rate of p53 protein in laryngeal carcinoma (56.8%, 25/44), all vocal cord polyps were negative; (3) HPV16 / 18 hybridization and p53 protein in laryngeal squamous cell carcinoma were both positive for 12/44, accounting for 63.2% of HPV16 / 18 hybrid positive specimens / 19), accounting for 48.0% (12/25) of p53 protein positive reaction. The results showed that HPV16 / 18 infection and p53 protein expression are closely related to the occurrence of laryngeal carcinoma, and the two may have a synergistic effect.