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本实验利用小鼠腹水型肝癌(H_(22))的肺转移倾向亚株(H_(22)SP_(10))与淋巴结转移倾向亚株(H_(22)SL_(10))和小鼠的心、肺、肝、肾、淋巴结冰切组织于体外混合培养,观察了不同转移能力的肿瘤细胞与正常组织间的相互作用。于混合培养24、48h后,计数冰切组织上粘附的肿瘤细胞,肝、肾、淋巴结上粘附的H_(22)细胞多于其它脏器。H_(22)SP_(10)主要粘附于肝、肾、肺,而H_(22)SL_(10)则主要粘附于淋巴结,几乎不粘附于肺组织。混合培养后的生长曲线表明加入的正常组织对三种瘤细胞生长具有不同作用,加入的五种组织对H_(22)生长均有促进作用,加入心、肝、肾组织使H_(22)SP_(10)细胞于培养48h之前呈递增趋势,而加入肺、淋巴结组织使H_(22)SP_(10)细胞生长数低于对照组,加入心脏组织96h后H_(22)SL_(10)细胞数开始高于对照组,而加入其它组织均使H_(22)SL_(10)细胞数低于对照组。该结果表明在活体中呈器官倾向性转移的瘤细胞在体外具有与相应组织特异性粘附的能力,而且这些细胞与不同宿主组织间存在不同相互作用。
In this study, mice with ascites-type hepatocellular carcinoma (H_(22)) were used as metastasis-prone sub-strains (H_(22)SP_(10)) and lymph node metastasis-prone sub-strains (H_(22)SL_(10)) and mice. Heart, lung, liver, kidney, and lymph node tissues were cultured in vitro and the interactions between tumor cells and normal tissues with different metastatic capacities were observed. After 24 and 48 hours of mixed culture, tumor cells adhering to ice-cut tissue were counted, and H_(22) cells attached to liver, kidney and lymph nodes were more than other organs. H_(22)SP_(10) mainly adheres to liver, kidney and lung, while H_(22)SL_(10) mainly adheres to lymph nodes and almost does not adhere to lung tissue. The growth curve after mixed culture showed that the added normal tissues had different effects on the growth of the three types of tumor cells, and the five added tissues promoted the growth of H_(22), and the H_(22)SP_ was added to the heart, liver and kidney tissues. (10) The cells showed an increasing trend before cultured for 48 h, while the addition of lung and lymph node tissues resulted in a lower growth of H_(22)SP_(10) cells than in the control group, and the number of H_(22)SL_(10) cells after 96 h of heart tissue addition. Compared with the control group, the number of H_(22)SL_(10) cells was lower than that of the control group. This result indicates that tumor cells metastasized to organs in vivo in vivo have the ability to specifically adhere to corresponding tissues in vitro, and that these cells have different interactions with different host tissues.