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目的 建立人体血浆中冰片的含量测定方法。方法 血浆样品用正已烷 -二氯甲烷 (9∶1)萃取 ,采用毛细管气相色谱法 ,色谱柱为CBP - 2 0 -M2 5 - 0 2 5石英弹性毛细管柱 ,以萘为内标进行测定。结果 冰片血浆浓度在 0 .0 5～ 5 .0 μg·ml-1范围内线性关系良好 ,r =0 .9997,最低检测浓度为 0 .0 2 μg·ml-1,平均萃取回收率为 83.0 9% ,平均方法回收率为 96 .5 5 % ,日内RSD <4 % ,日间RSD <6 %。结论 本法简便灵敏、准确可靠 ,可作为人体血浆中冰片的含量测定方法。
Objective To establish a method for the determination of borneol in human plasma. Methods The plasma samples were extracted with n - hexane - methylene chloride (9: 1), and were characterized by capillary gas chromatography with a CBP - 2 0 -M2 5 - 0 2 5 quartz capillary column and naphthalene as an internal standard . Results The linear relationship between borneol concentration in the range of 0.5-0.5 μg · ml-1 was good, r = 0.9997, the lowest detection concentration was 0. 02 μg · ml-1, and the average extraction recovery was 83.0 9%. The average recovery rate was 96.55%. The intraday RSD was less than 4% and the intraday RSD was less than 6%. Conclusion This method is simple and sensitive, accurate and reliable, and can be used as a method for the determination of borneol in human plasma.