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目的:研究血清应答因子(serum response factor,SRF)在人食管鳞癌细胞体外侵袭转移中的意义。方法:选用EC9706-H、EC9706-L和EC109-H、EC109-L两对高低转移细胞系,采用细胞划痕实验验证食管鳞癌高低转移细胞系体外侵袭转移能力的差异;Western blot检测SRF在两对食管鳞癌高低转移细胞系中的差异表达;在EC9706-H、EC109-H细胞中加入CCG(SRF抑制剂)抑制SRF的表达后,检测其侵袭转移能力的变化。结果:细胞划痕实验验证了两对食管鳞癌高低转移细胞系侵袭转移能力的差异;Western blot结果提示SRF在EC9706-H、EC109-H细胞中的表达水平显著高于EC9706-L、EC109-L细胞;在EC9706-H、EC109-H细胞中加入CCG抑制SRF的表达后,其侵袭转移能力明显减退。结论:SRF在高转移性食管鳞癌细胞系中呈现高表达,在低转移性食管鳞癌细胞系中呈现低表达,抑制高转移性食管鳞癌细胞系中SRF的表达后,其侵袭转移能力下降,提示SRF和食管鳞癌的侵袭转移能力呈正相关。
Objective: To investigate the significance of serum response factor (SRF) in the invasion and metastasis of human esophageal squamous cell carcinoma in vitro. Methods: EC9706-H, EC9706-L, EC109-H and EC109-L two pairs of high and low metastatic cell lines were used to detect the difference of invasion and metastasis of esophageal squamous cell carcinoma cell lines in vitro using cell scratch assay. Two pairs of esophageal squamous cell carcinoma metastasis of high and low metastatic cell lines in the expression of different; EC9706-H, EC109-H cells by adding CCG (SRF inhibitor) inhibition of SRF expression, detection of invasion and metastasis changes. Results: The cell scratch assay verified the difference of invasion and metastasis of esophageal squamous cell carcinoma cell lines between two groups. Western blot results showed that the expression level of SRF in EC9706-H and EC109-H cells was significantly higher than that of EC9706-L and EC109- L cells. The expression of SRF was inhibited by CCG in EC9706-H and EC109-H cells. CONCLUSIONS: SRF is highly expressed in esophageal squamous cell carcinoma cell line with high metastasis, low expression in low metastatic esophageal squamous cell carcinoma cell line, and inhibits SRF expression in esophageal squamous cell carcinoma cell line with high metastatic potential Decreased, suggesting that SRF and esophageal squamous cell carcinoma invasion and metastasis was positively correlated.