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建立了一种用纤维素手性固定相拆分消旋卡多曲光学异构体的高效液相色谱方法。采用纤维素-三(3,5-二甲基苯胺基甲酸酯)手性固定相(Chiralpak IB),考察醇类添加剂种类及比例、柱温和流速对拆分的影响。流动相为正己烷-异丙醇(60︰40),流速0.6 ml/min,柱温25℃,检测波长210 nm,卡多曲两个异构体在Chiralpak IB手性柱上能够达到基线分离,在0.01～0.5 mg/ml线性关系良好,平均回收率为99.7%和99.6%,RSD为0.79%和0.91%。
A high performance liquid chromatography (HPLC) method was developed for the resolution of racemic card multifunctional optical isomers using cellulose chiral stationary phase. Cellulose tris (3,5-dimethylphenylcarbamate) chiral stationary phase (Chiralpak IB) was used to investigate the influence of alcohol type and proportion, column temperature and flow rate on the resolution. The mobile phase was n-hexane-isopropanol (60:40), the flow rate was 0.6 ml / min, the column temperature was 25 ℃ and the detection wavelength was 210 nm. The two isomers of carduole could be separated on Chiralpak IB chiral column The linearity was good at 0.01-0.5 mg / ml. The average recoveries were 99.7% and 99.6% with RSDs of 0.79% and 0.91%, respectively.