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以沟叶结缕草匍匐茎诱导的胚性愈伤组织为材料,通过连续3代的培养,比较了MS、N6和N6AA3种基本培养基与不同浓度(0.5、1.0、1.5、2.0mg·L-1)2,4-D组合对愈伤组织生长速度、胚性保持、再生能力、再生植株的抗氧化酶活性、脯氨酸含量和染色体倍性的影响。结果表明:MS培养基添加2.0mg·L-12,4-D对愈伤组织的生长和胚性保持效果最好,胚性愈伤组织比率达85.00%。但不同基本培养基对再生植株的SOD、POD、CAT活性和脯氨酸含量没有显著影响,流式细胞仪测定结果表明其染色体倍性也没有发生变化,植株个体间没有形态差异。
The embryogenic callus induced by stolonifer of Zoysia matrella was used as materials. The three kinds of basic media of MS, N6 and N6AA were compared with those of different concentrations (0.5, 1.0, 1.5 and 2.0 mg · L- 1) Effect of 2,4-D on callus growth rate, embryogenic maintenance, regeneration ability, antioxidant enzyme activity, proline content and chromosome ploidy of regenerated plants. The results showed that MS medium supplemented with 2.0 mg · L-12,4-D had the best effect on the growth and embryogenic maintenance of callus, with the rate of embryogenic callus reaching 85.00%. However, different basic medium had no significant effect on the activities of SOD, POD and CAT and the content of proline in regenerated plants. The results of flow cytometry showed that the chromosome ploidy did not change, and there was no morphological difference among the plants.