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作者使用 0 .3% Triton X- 1 0 0、1 .5 % 3-〔(3-胆酰胺丙基 ) -二乙铵〕-丙磺酸 (CHAPS)和 1 5 %十二烷基硫酸钠 (SDS)作为预处理液 ,取 5 0 μl与 1 0 0 μl标本混合 ,5 6℃作用 30分钟进行预处理。同时 ,在大肠杆菌中表达重组丙型肝炎病毒 (HCV)核心抗原融合蛋白(HCV基因型
The authors used 0.3% Triton X-100, 0.5% 3 - [(3-cholamidopropyl) -dimethylammonium] -propanesulfonic acid (CHAPS) and 15% sodium dodecyl sulfate (SDS) as the pretreatment solution, take 50 μl mixed with 100 μl of the sample and pretreated at 56 ° C for 30 minutes. In the meantime, a recombinant hepatitis C virus (HCV) core antigen fusion protein (HCV genotype