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目的探讨氧化应激在三氯乙烯(trichloroethylene,TCE)致敏豚鼠肝损伤中的作用。方法选用体重250~300 g的白色雌性豚鼠,随机分成空白对照组,溶剂(橄榄油)对照组,TCE处理组。根据豚鼠最大值实验(guinea pig maximization test,GPMT)方法处理豚鼠。按照《化学品毒性鉴定技术规范》的评分标准对动物的皮肤反应进行评分,评分≥1的判为致敏。在终末激发后24 h和72 h取材,对肝组织进行病理学检查,并分别测定血清中丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)和白蛋白(ALB)和肝脏中的丙二醛(MDA)含量、超氧化物歧化酶(SOD)活力。结果TCE处理组致敏率为65.38%。TCE致敏72 h组中见到较多肝细胞水肿,并有胞核破裂消失。TCE致敏72 h组ALT和AST水平比TCE致敏24 h组和TCE未致敏72 h组升高,差异有统计学意义(P<0.05);TCE致敏72 h组ALB水平比溶剂对照组和TCE未致敏72 h组降低,差异有统计学意义(P<0.05)。TCE致敏24 h和72 h组肝MDA含量与溶剂对照组相比明显升高,且差异有统计学意义(P<0.05);TCE致敏24 h组肝SOD活力与溶剂对照组相比较明显降低,差异有统计学意义(P<0.05),而TCE致敏72 h组肝SOD活力与溶剂对照组相比较明显升高,差异有统计学意义(P<0.05)。结论TCE可引起致敏豚鼠肝损伤,氧化应激在肝损伤中发挥一定的作用。
Objective To investigate the role of oxidative stress in hepatic injury induced by trichlorethylene (TCE) in guinea pigs. Methods White female guinea pigs weighing 250-300 g were randomly divided into blank control group, solvent (olive oil) control group and TCE treatment group. Guinea pigs were treated according to the guinea pig maximization test (GPMT) method. Animal skin reactions were scored according to the scoring criteria of the Technical Specifications for Toxicity Identification of Chemicals and the score ≥1 was judged as sensitization. Pathological examination was performed at 24 h and 72 h after the final challenge, and serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and albumin (ALB) were measured ) And liver malondialdehyde (MDA) content, superoxide dismutase (SOD) activity. Results The sensitization rate of TCE group was 65.38%. TCE sensitized 72 h group to see more hepatocyte edema, and rupture of nucleus disappeared. The level of ALT and AST in 72 h TCE sensitized group was higher than that in TCE sensitized 24 h and TCE unsensitized 72 h group (P <0.05) Group and TCE non-sensitized 72 h group decreased, the difference was statistically significant (P <0.05). Compared with the control group, the content of MDA in liver tissue of TCE-sensitized group was significantly increased at 24 and 72 h (P <0.05). Compared with the solvent control group, (P <0.05). Compared with the solvent control group, the activity of SOD in the liver of TCE-sensitized group at 72 h was significantly higher than that of the control group (P <0.05). Conclusion TCE can cause liver injury in sensitized guinea pigs and oxidative stress play a role in liver injury.