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目的:加强综合性医院痰涂片镜检的质量控制,保证痰涂片检验质量,提高痰涂片阳性检出率。方法:采用痰直接涂片萋尼氏染色检查法,根据《中国结核病防治规划-痰涂片镜检实验室质量保证手册》的规定进行涂片、染色和镜检。结果:痰检阳性、阴性符合率超过99%;痰细胞合格率为90.12%～95.34%,痰涂片涂抹大小、厚薄、染色、脱落等合格率呈增长趋势,基本超过项目要求的90%;血性痰涂阳率>干酪痰涂阳率>黏液痰涂阳率>水样痰涂阳率;清晨痰涂阳率>夜间痰涂阳率>即时痰涂阳率。结论:痰检阳性、阴性符合率、痰片制作各项指标基本达到项目要求,血性痰和清晨痰的涂阳率最高。
Objective: To strengthen the quality control of sputum smear microscopy in general hospitals, to ensure the quality of sputum smear test and to improve the positive detection rate of sputum smear. Methods: Sputum direct smear 萋 Nisshin staining test method, according to “China Tuberculosis Prevention and Control Program - Sputum smear microscopy laboratory quality assurance manual,” the provisions of smear, staining and microscopic examination. Results: The coincidence rate of positive sputum test and negative test was over 99%. The passing rate of sputum cells was 90.12% ~ 95.34%. The pass rate of sputum smear smearing size, thickness, dyeing and shedding showed an increasing trend, basically exceeding 90% of the project requirements. Bloody sputum smear positive rate> sputum smear positive rate> mucus sputum smear rate> water sputum smear rate; morning sputum smear rate> night sputum smear rate> instant sputum smear rate. Conclusion: The sputum examination positive, negative coincidence rate, sputum production of the basic indicators to meet the project requirements, bloody sputum and sputum sputum smear rate highest.