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为探索瑞香狼毒(Stellera chamaejasme)提取物对草莓灰霉病菌(Botrytis cinerea)的抑菌活性,采用生物活性跟踪法对瑞香狼毒的抑菌活性成分进行初步研究,柱层析法对其甲醇萃取物高活性成分进行分离,经LC-MS和核磁共振仪对最终成分进行结构鉴定,结果表明瑞香狼毒最高活性流份3的主要抑菌活性成分为伞形花内酯,并且随着浓度的提高,该活性成分对草莓灰霉病菌的抑制作用也增强,其抑菌中浓度为1.52 mg.mL-1。通过扫描电镜观察流份3对草莓灰霉病菌超微结构的影响,结果发现伞形花内酯严重破坏了草莓灰霉病菌孢子和菌丝体的形态结构。
In order to explore the antibacterial activities of extracts of Stellera chamaejasme against Botrytis cinerea, the antibacterial activity of Stellera chamaejasme against Stellera chamaejasme was preliminary studied by bioactivity tracing method. The results showed that the main antibacterial activity component of the highest active fraction 3 of Stellera chamaejasme is Uvagonone, and with the concentration The inhibitory effect of the active ingredient against Botrytis cinerea also increased, and the bacteriostatic concentration was 1.52 mg.mL-1. Scanning electron microscopy (FACS) was used to observe the effect of Fraction 3 on the ultrastructure of Botrytis cinerea. The results showed that Umbelliferone severely damaged the morphological structure of the spores and mycelia of B. cinerea.