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目的探讨子宫内膜癌组织中长链非编码RNA MALAT1的表达及临床意义。方法采用实时荧光定量聚合酶链反应(quantitative real-time polymerase chain reaction,qRT-PCR)方法检测榆林市神木县医院2009年9月至2010年9月确诊的104例子宫内膜癌患者新鲜子宫内膜癌组织标本及其对应的癌旁2 cm组织标本中MALAT1的表达,分析其与子宫内膜癌的临床病理特征关系以及诊断价值和预后关系。结果 MALAT1在子宫内膜癌组织中的表达明显高于子宫内膜癌旁组织(P<0.05);MALAT1在子宫内膜癌高、中分化组和低分化组中的阳性表达差异无统计学意义(P>0.05);MALAT1在子宫内膜癌组织中的表达与淋巴结转移、肿瘤浸润深度以及血管侵袭相关,差异均有统计学意义(P<0.05);ROC曲线AUC=0.7314(95%CI,0.6141~0.8487;P<0.05);MALAT1高表达和低表达组在总生存时间OS(χ2=7.269,P<0.05)及无进展生存时间PFS(χ2=5.182,P<0.05)上的差异有统计学意义。结论长链非编码RNA MALAT1在子宫内膜癌中是高表达的,可以作为子宫内膜癌的新型生物标记物和诊断靶标。
Objective To investigate the expression of long chain noncoding RNA MALAT1 in endometrial carcinoma and its clinical significance. Methods Quantitative real-time polymerase chain reaction (qRT-PCR) was used to detect the fresh uterus in 104 cases of endometrial cancer diagnosed in Shenmu County Hospital of Yulin City from September 2009 to September 2010 The correlation between the expression of MALAT1 and the clinicopathological characteristics of endometrial carcinoma and the relationship between the diagnostic value and prognosis was analyzed. Results The expression of MALAT1 in endometrial carcinoma was significantly higher than that in endometrial carcinoma (P <0.05). The positive expression of MALAT1 in high, moderately differentiated and poorly differentiated endometrial carcinoma was not statistically significant (P> 0.05). The expression of MALAT1 in endometrial carcinoma was correlated with lymph node metastasis, tumor invasion depth and vascular invasion, the difference was statistically significant (P <0.05). The AUC of ROC curve was 0.7314 (95% CI, 0.6141 ~ 0.8487; P <0.05). There was statistically significant difference in the total survival time OS (χ2 = 7.269, P <0.05) and progression-free survival time PFS Significance of learning. Conclusion The long-chain non-coding RNA MALAT1 is highly expressed in endometrial carcinoma and can be used as a new biomarker and diagnostic target for endometrial cancer.