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[Objective]To compare the preservation effects of three long-term preservation methods on inhibiting the metabolic activity of Cordyceps sinensis culture.[Method] Taking the growth of mycelium,polysaccharide of and cordycepic acid as indexes to evaluate quality of Cordyceps sinensis,the effects of sharp freezing,liquid nitrogen freezing and freeze-drying on conserving Cordyceps sinensis culture of C-03 were comparatively studied.[Result]The Cordyceps sinensis culture through vacuum freeze-drying preservation due to skimmed milk and phased cooling of 4 ℃ for 30 min,-20 and-80 ℃ freezing for 2 and 24 h respectively was growing well when germination,the yield of mycelial growth and active ingredients were relatively higher than the other two methods.[Conclusion]Vacuum freeze-drying preservation was the desirable method to long-term preserve Cordyceps sinensis culture.
[Objective] To compare the preservation effects of three long-term preservation methods on inhibiting the metabolic activity of Cordyceps sinensis culture. [Method] Taking the growth of mycelium, polysaccharide of and cordycepic acid as indexes to evaluate quality of Cordyceps sinensis, the effects [Result] The Cordyceps sinensis culture through vacuum freeze-drying preservation due to skimmed milk and phased cooling of 4 ° C for 30 min , -20 and -80 ℃ freezing for 2 and 24 h respectively were growing well when germination, the yield of mycelial growth and active ingredients were relatively higher than the other two methods. [Conclusion] Vacuum freeze-drying preservation was the desirable method to long-term preserve Cordyceps sinensis culture.