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目的:制备地高辛标记的PTA1基因探针,以用于PTA1表达及功能的研究.方法:根据新克隆的人PTA1基因序列,设计针对PTA1分子不同结构域的引物,通过PCR扩增、地高辛标记的方法制备基因探针.结果:细胞原位杂交方法证实这种探针具有较高的敏感性和特异性.结论:PTA1基因探针的制备为进一步研究PTA1分子的分布、表达及功能打下了较好的基础.
Objective: To prepare digoxigenin-labeled PTA1 gene probe for the study of PTA1 expression and function. Methods: According to the newly cloned human PTA1 gene sequence, primers designed for different domains of PTA1 molecule were designed and gene probes were prepared by PCR amplification and digoxigenin labeling. Results: In situ hybridization proved that this probe has high sensitivity and specificity. Conclusion: The preparation of PTA1 gene probe laid a good foundation for further study on the distribution, expression and function of PTA1.