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目的 探讨食管鳞癌ＤＮＡ异倍体和ＲＮＡ含量变化与预后的关系。方法 应用流式细胞技术对６２例随访患者的石蜡标本进行研究。结果 术后生存５年以上患者的ＤＮＡ异倍体７８．１％（２５／３２），ＲＮＡ含量为２０．５３±４．２１。术后半年内死亡者ＤＮＡ为９３．３％ （２８／３０），ＲＮＡ含量为２４．８４±６．３５，两者均有显著性差异。ＤＮＡ异倍体和ＲＮＡ含量与病期、恶性程度和淋巴结转移有关。结论 ＤＮＡ和ＲＮＡ测定是判断食管鳞癌病期、恶性程度、有无淋巴结转移和预后的重要指标。表２ 食管癌不同病期与ＤＮＡ和ＲＮＡ含量的关系生存时间（年） 病期 例数 ＤＮＡ２Ｃ Ａｎ ％ＲＮＡ含量（ｘ±ｓ）＞ ５ Ⅰ ３ ２ １ ５０ １０．６１±０．２４Ⅱ １９ ４ １５ ７８．９ ２１．５６±３．４１Ⅲ ８ １ ７ ８７．５ ２３．９８±２．１１Ⅳ ２ ０ ２ １００ ２６．１２±１．５６＜ ０．５ Ⅰ ０ ０ ０Ⅱ １１ １ １０ ９０．９ ２３．４５±４．２７Ⅲ １３ １ １２ ９２．３ ２４．７６±２．８７Ⅳ ６ ０ ６ １００ ２７．３１±２．５６表３ 食管鳞病分化程度与ＤＮＡ和ＲＮＡ含量的关系生存时间（年） 分化 例数 ＤＮＡ２Ｃ Ａｎ ％ＲＮＡ含量（ｘ±ｓ）＞ ５ Ⅰ ５ ３
Objective To investigate the relationship between changes of DNA aneuploidy and RNA content and prognosis in esophageal squamous cell carcinoma. Methods Paraffin-embedded specimens of 62 patients were studied by flow cytometry. Results The DNA aneuploidy was 78.1% (25/32) in patients who survived more than 5 years after surgery, and the RNA content was 20.53±4.21. In the six months after the death, the DNA of the dead was 93.3% (28/30), and the RNA content was 24.84±6.35. There was a significant difference between the two groups. DNA aneuploidy and RNA content are associated with stage, degree of malignancy, and lymph node metastasis. Conclusion DNA and RNA measurement is an important indicator to determine the stage, degree of malignancy, lymph node metastasis and prognosis of esophageal squamous cell carcinoma. Table 2 Relationship between different stages of esophageal cancer and DNA and RNA content Survival time (years) Case number DNA2C An %RNA content (x±s)> 5 I 3 2 1 50 10.61±0.24II 19 4 15 78.9 21.56±3.41III 8 1 7 87.5 23.98±2.11IV 2 0 2 100 26.12±1.56< 0.5 I 0 0 0II 11 1 10 90.9 23 .45±4.27III 13 1 12 92.3 24.76±2.87IV 6 0 6 100 27.31±2.56 Table 3 Relationship between esophageal squamous cell differentiation and DNA and RNA content Survival time (years) Differentiation Number of cases DNA2C An %RNA content (x±s)> 5 I 5 3