[Objective] To observe the protective effect of swertiamarin on H2O2-induced apoptosis of mice cardiomyocytes and explore its protective mechanism.[Methods] Cultured with selective plating method,the cell viability,SOD and MDA contents of SD mice cardiomyocyte were determined;the expression rates of apoptosis proteins bcl-2 and Bax were detected with immunocytochemical technique,and the cell apoptosis was detected by acridine orange fluorescent staining.[Results] The viability and SOD activity of cardiomyocytes in medicine groups with different concentrations were all improved,and the content of MDA was lowered as well,which had significant differences with that in H2O2-induced group(P<0.05;P<0.01).[Conclusion] Swertiamarin can protect the H2O2-induced cardiomyocytes effectively,and the protective mechanism may be related to the oxidation system and the regulation of specific proteins. [Objective] To observe the protective effect of swertiamarin on H2O2-induced apoptosis of mice cardiomyocytes and explore its protective mechanism. [Methods] Cultured with selective plating method, the cell viability, SOD and MDA contents of SD mice cardiomyocyte were determined; the expression rates of apoptosis proteins bcl-2 and Bax were detected with immunocytochemical technique, and the cell apoptosis was detected by acridine orange fluorescent staining. [Results] The viability and SOD activity of cardiomyocytes in medicine groups with different concentrations were all improved, and the content of MDA was lowered as well, which had significant differences with that in H2O2-induced group (P <0.05; P <0.01). [Conclusion] Swertiamarin can protect the H2O2-induced cardiomyocytes effectively, and the protective mechanism may be related to the oxidation system and the regulation of specific proteins.