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目的研究Ｍｒ７２０００Ⅳ型胶原酶（ＭＭＰ＿２）在肝癌细胞中的表达与肝癌侵袭转移的关系．方法以原位分子杂交及免疫组化方法检测３４例原发性肝癌及其癌旁组织中ＭＭＰ＿２ｍＲＮＡ及其蛋白的表达．患者３０例伴肝硬变，４例无肝硬变，其中２４例肝癌突破包膜向周围肝组织侵袭性生长、门静脉癌栓形成或形成转移灶，１０例肝癌包膜完整．结果呈侵袭性生长的２４例肝癌中１６例癌细胞ＭＭＰ＿２ｍＲＮＡ表达明显增高，而包膜完整的１０例肝癌中仅有２例ＭＭＰ＿２ｍＲＮＡ表达阳性．侵袭性生长的肝癌２４例中１８例ＭＭＰ＿２呈不同程度的阳性表达，而包膜完整的肝癌１０例中仅３例ＭＭＰ＿２表达阳性，向外突出生长的肝癌细胞ＭＭＰ＿２表达阳性，转移组肝癌（发生门静脉癌栓或转移）ＭＭＰ＿２阳性率（８３３％，１５／１８）明显高于未转移组肝癌ＭＭＰ＿２阳性率（３７５％，６／１６）（Ｐ＜００１）．结论由肝癌细胞产生的ＭＭＰ＿２与肝癌的侵袭转移密切相关．
Objective To study the relationship between the expression of Mr72000 type IV collagenase (MMP_2) in hepatocellular carcinoma and the invasion and metastasis of hepatocellular carcinoma. Methods In situ hybridization and immunohistochemistry were used to detect the expression of MMP-2 mRNA and protein in 34 cases of primary hepatic carcinoma and its adjacent tissues. Thirty patients had liver cirrhosis and 4 had no liver cirrhosis. Among them, 24 cases of liver cancer broke through the capsule to invade surrounding hepatic tissue, formed portal vein tumor emboli or formed metastatic foci, and 10 cases of liver cancer had complete capsule. Results The expression of MMP 2 mRNA in 16 cases of invasive liver cancer was significantly increased in 16 cases, while only 2 cases of intact 10 cases of liver cancer had positive expression of MMP 2 mRNA. Among the 24 cases of invasive hepatocellular carcinoma, 18 cases had positive expression of MMP 2 in 18 cases, while only 10 out of 10 cases of intact hepatocellular carcinoma had positive expression of MMP 2 . The positive expression of MMP 2 was positive in the outgrowth of hepatocellular carcinoma cells. The positive rates of MMP-2 in portal vein tumor thrombus or metastasis were significantly higher than those in non-metastatic liver cancer (83. 3%, 15/18) (37.5%, 6/16) (P<0. 01). Conclusion MMP_2 produced by hepatoma cells is closely related to the invasion and metastasis of hepatocellular carcinoma.