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目的观察大鼠单肺移植缺血再灌注肺损伤肺功能改变及姜黄素(CUR)干预作用。方法建立大鼠原位异体左单肺移植模型。纯种雄性SD大鼠120只,随机分两组,每组又分三个亚组(n=12):假手术组、载体组、CUR组,分别在缺血4 h、再灌注2 h和24 h处死大鼠,测量移植肺损伤病理评分、氧合指数、肺湿干重比(W/D)、伊文思篮染料(EBD)含量。结果移植肺组织病理学主要表现为肺水肿,炎症细胞浸润,出血。与假手术组比较,再灌注2 h,移植肺氧合指数从358.3下降到153.7,W/D从3.7增加到5.6,EBD含量从381.0μg/g干重增加到1172.3μg/g干重;再灌注24 h后,氧合指数从394.2下降到102.3,移植肺W/D从3.8增加到6.6;EBD含量从387.5μg/g干重增加到927.5μg/g干重。供体和受体大鼠经CUR预处理后,明显减少缺血4 h,再灌注2和24 h的移植肺组织学病理评分,肺泡毛细血管通透性,湿干重比,改善气体交换。结论大鼠单肺移植后缺血再灌注损伤(IRI)肺功能学改变主要表现为肺泡毛细血管内皮损伤导致的通透性肺水肿及由此导致的氧合功能下降。CUR对大鼠单肺移植缺血再灌注肺损伤具有保护作用。
Objective To observe the changes of lung function in rats with ischemia-reperfusion lung injury and the curative effect of curcumin (CUR). Methods Rat orthotopic allogeneic left lung transplantation model was established. 120 purebred male Sprague-Dawley rats were randomly divided into two groups. Each group was divided into three subgroups (n=12): sham operation group, vehicle group, and CUR group, 4 h after ischemia and 2 h after reperfusion respectively. The rats were sacrificed at 24 h and pathological scores of lung injury, oxygenation index, wet/dry lung weight ratio (W/D), and Evans basket dye (EBD) were measured. Results The histopathology of transplanted lung mainly manifested as pulmonary edema, inflammatory cell infiltration and hemorrhage. Compared with the sham group, reperfusion 2 h, the graft oxygenation index decreased from 358.3 to 153.7, W/D increased from 3.7 to 5.6, EBD content increased from 381.0μg/g dry weight to 1172.3μg/g dry weight; After 24 h of infusion, the oxygenation index decreased from 394.2 to 102.3, the transplanted lung W/D increased from 3.8 to 6.6, and the EBD content increased from 387.5 μg/g dry weight to 927.5 μg/g dry weight. Pretreatment with CUR in donor and recipient rats significantly reduced the histological pathology score, alveolar capillary permeability, and wet-to-dry weight ratio of the transplanted lung at 4 h, 2 h and 24 h after reperfusion, and improved gas exchange. Conclusions The changes of pulmonary function after ischemia-reperfusion injury (IRI) in rats after single lung transplantation are mainly caused by pulmonary edema caused by pulmonary capillary endothelium injury and decreased oxygenation. CUR has a protective effect on rat lung ischemia-reperfusion injury.