论文部分内容阅读
为了给表达广谱抗真菌蛋白转基因水稻的安全性评估提供基础数据,本文以表达广谱抗真菌蛋白转基因水稻转品1和转品8及非转基因七丝软粘(对照)为试材,对其秸秆化学成分进行分析,同时,采用傅立叶变换红外光谱(FTIR)和X-射线衍射(XRD)方法观察秸秆中不同部位纤维素结构的变化情况。研究结果显示:(1)转基因水稻转品1和转品8与对照在秸秆相同部位的化学成分含量不存在显著差异;(2)转基因水稻转品1和转品8及对照在秸秆相同部位纤维素的红外吸收峰形状基本一致,且品种间的吸收峰强度和结晶指数(O’KI和N·O’KI)也无显著差异;(3)转品1和转品8秸秆的X-射线衍射图与对照相似,且结晶度(CrI)与对照无显著差异。综上所述,广谱抗真菌基因的导入不会对水稻的化学成分、纤维素结构及晶体结构产生明显影响。
In order to provide basic data for the safety evaluation of transgenic rice expressing broad-spectrum antifungal protein, we used the transgenic rice transgenic plants 1 and 6 and non-transgenic 7-filament soft bone (control) The chemical constituents of straw were analyzed. At the same time, the changes of cellulose structure in different parts of straw were observed by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The results showed that: (1) there was no significant difference in the chemical components of the same parts of the transgenic rice; (2) The transgenic rice products 1 and 6 and the control had the same fiber content in the same part of the straw The infrared absorption peak shape of the prime is basically the same, and there is no significant difference between the absorption peak intensity and the crystal index (O’KI and N · O’KI) between the varieties; (3) X-ray The diffraction pattern is similar to the control, and the crystallinity (CrI) is not significantly different from the control. In summary, the introduction of broad-spectrum antifungal genes will not have a significant impact on the chemical composition of rice, cellulose structure and crystal structure.