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目的和方法：采用蛋白激酶Ｃ（ＰＫＣ）催化区抑制剂ｓｔａｕｒｏｓｐｏｒｉｎｅ（ＳＴ）、调节区抑制剂ｓｐｈｉｎｇｏｓｉｎｅ（ＳＳ），诱导ＣＮＥ—２Ｚ细胞２４ｈ。结果：（１）ＳＴ、ＳＳ对细胞ＤＮＡ裂解的影响均随浓度增高而逐步增强，作用明显的终浓度分别为１×１０６ｍｏｌ／Ｌ和４×１０５；（２）核形态观察：诱导后部分细胞核变小，核浓缩及核碎裂；（３）ＤＮＡ琼脂糖电泳：诱导后的细胞有典型梯状ＤＮＡ条带；（４）流式细胞仪分析：诱导组Ｇ１期前均有ＤＮＡ亚二倍体峰，即凋亡峰。细胞周期改变：ＳＴ使Ｇ２期增加、Ｇ１、Ｓ期减少；ＳＳ使Ｓ期增加和Ｇ１期减少。结论：ＰＫＣ抑制剂可有效地促进ＣＮＥ－２Ｚ细胞凋亡，但与抑制剂剂量有关，细胞周期的改变可能在ＰＫＣ抑制剂诱导的细胞凋亡中起重要作用。
Objective and Methods: To induce CNE-2Z cells for 24 h with staurosporine (ST), the inhibitor of protein kinase C (PKC), and sphingosine (SS), the inhibitor of the regulatory region. Results: (1) The effects of ST and SS on the DNA cleavage of cells were gradually increased with the increase of the concentration. The obvious final concentrations were 1×10-6mol/L and 4×10-5, respectively; (2) Observation of nuclear morphology: After induction, part of the nuclei became smaller, nuclear condensation and nuclear fragmentation; (3) DNA agarose electrophoresis: cells with typical ladder-like DNA bands after induction; (4) flow cytometry analysis: the induction group had all before G1 phase The DNA hypodiploid peak, the apoptotic peak. Changes in cell cycle: ST increased G2 and decreased G1 and S phases; SS increased S phase and decreased G1 phase. Conclusion: PKC inhibitor can effectively promote the apoptosis of CNE-2Z cells, but it is related to the dose of inhibitor. The change of cell cycle may play an important role in the apoptosis induced by PKC inhibitors.