应用ＭＨ６０·ＢＳＦ２细胞检测ＩＬ－６生物学效价时，存在该细胞饲养困难和长期培养容易变异而失去ＩＬ－６增殖依赖性等问题。为此我们首先探讨了克隆化筛选ＭＨ６０·ＢＳＦ２的方法，从增殖依赖性较差的ＭＨ６０·ＢＳＦ２细胞群体中筛选到３株ＩＬ－６强依赖细胞株。并探讨了该细胞的冻存复苏方法。所冻存的细胞半年以上亦无ＩＬ－６增殖依赖性的改变。在此基础上采用ＭＴＴ比色法取代［￣３Ｈ］ＴｄＲ掺入法获得成功，从而使ＩＬ－６待测标本的生物活性检测更为方便经济。 When detecting the biological potency of IL-6 using MH60 · BSF2 cells, there are some problems such as difficulty in raising the cells, easy mutation of the cells in long-term culture, and loss of dependence on IL-6 proliferation. To this end, we firstly discussed the method of clonal screening of MH60 · BSF2, and screened three strong IL-6-dependent cell lines from MH60 · BSF2 cell population with poor proliferation. And explored the cell cryopreservation method. The frozen cells more than six months nor IL-6 proliferation-dependent changes. On this basis, the MTT colorimetric method was used to replace [¯3H] TdR incorporation, which made the detection of biological activity of IL-6 samples more convenient and economical.