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目的建立使用小鼠神经母细胞瘤Neuro-2a细胞替代原代培养神经元进行神经轴突测量的方法,用于神经损伤研究。方法使用浓度200μmol/L、500μmol/L、1 000μmol/L过氧化氢处理Neuro-2a细胞12h,戊二醛固定,生物染色,在光学显微镜下手动测量突起长度。结果过氧化氢可剂量依赖性引起神经突起逐渐回缩变短,1 000μmol/L过氧化氢处理组光镜下见不到明显突起,突起测量数据显示,过氧化氢处理后突起长度与未经处理细胞有显著差异。结论 Neuro-2a细胞在一定程度上可替代原代培养神经元进行轴突测量研究,该测量方法可用于神经轴突再生研究。
Objective To establish a method for neuronal axon measurement using mouse neuroblastoma Neuro-2a cells instead of primary cultured neurons for nerve injury studies. Methods Neuro-2a cells were treated with 200μmol / L, 500μmol / L and 1 000μmol / L hydrogen peroxide for 12h, fixed with glutaraldehyde, and stained with biotin. The length of the protuberances was measured under a light microscope. Results Hydrogen peroxide induced neurite outgrowth gradually in a dose-dependent manner. In the 1 000 μmol / L hydrogen peroxide treatment group, no obvious protuberances were observed under the light microscope. The measurement data of the protuberance showed that the length of the protuberance after the treatment with hydrogen peroxide There are significant differences between treated cells. Conclusion Neuro-2a cells can be used to replace the primary cultured neurons for axonal measurement to a certain extent. This method can be used to study axonal regeneration.