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利用原位ＤＮＡ末端转移酶（ＩＳＴｄＴ）法检测肝硬变、肝癌组织标本及小鼠小肠粘膜上皮细胞凋亡。结果发现２５ｍｇＬ－１蛋白酶Ｋ消化１５ｍｉｎ，ＴｄＴ反应后采用中火档微波处理５ｍｉｎ可获最佳结果。对上述条件的最佳选择进行了探讨
Liver cirrhosis, hepatocellular carcinoma and intestinal mucosal epithelial cell apoptosis were detected by in situ DNA terminal transferase (ISTdT) assay. The results showed that 25mgL-1 proteinase K digestion 15min, TdT reaction using medium fire file microwave 5min to get the best results. The best choice of the above conditions is discussed