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目的探讨逍遥散延缓兔膝关节软骨组织退行性变的可能作用机制。方法 40只新西兰兔通过手术方法进行膝关节失稳型造模,1周后随机分为中药组、西药组、混合组和对照组各10只,驱赶行走4周,分别灌药或灌生理盐水8周。造模后12周末处理动物,采用原位末端标记法检测兔膝关节软骨细胞凋亡及免疫组化法检测软骨增殖细胞核抗原的表达。结果中药组、西药组和混合组软骨细胞凋亡率均低于对照组;软骨增殖细胞核抗原的表达率均高于对照组。两组数据比较有统计学意义。结论逍遥散可明显降低兔膝关节软骨细胞凋亡及增强软骨细胞增殖,可能是逍遥散延缓兔膝关节软骨组织退行性变的机制之一。
Objective To investigate the possible mechanism of Xiaoyaosan delaying the degeneration of knee cartilage tissue. Methods Forty New Zealand rabbits were surgically performed knee instability modeling. One week later, 10 New Zealand rabbits were randomly divided into TCM group, western medicine group, mixed group and control group, each of which was driven for 4 weeks. The rabbits were drenched with saline or saline 8 weeks. Animals were sacrificed at 12 weeks after modeling. Apoptosis of rabbit articular chondrocytes was detected by in situ end-labeling, and proliferating cell nuclear antigen expression was detected by immunohistochemistry. Results The apoptosis rates of chondrocytes in Chinese medicine group, western medicine group and mixed group were lower than those in control group. The expression rates of proliferating cell nuclear antigen in cartilage were higher than those in control group. Two sets of data more statistically significant. Conclusion Xiaoyao powder can significantly reduce the chondrocyte apoptosis and increase the proliferation of chondrocytes in rabbits, which may be one of the mechanisms that Xiaoyaosan delays the degeneration of rabbit articular cartilage tissue.