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本文报道了中国特种稻（包稻）分子进化研究的初步结果。本研究利用分布于我国不同省份的有色稻品种,试图以酯酶同工酶和过氧化物酶同工酶的酶谱带为分子指标,运用分支分类学的基本原理,编制出 PAUP2.4. 1、PAUP30和 Hennig86三个计算机软件包,用手工操作法对酯酶同工酶谱带进行分析和分子进化树的制作。研究结果表明,酯酶同工酶中1、4、7类型为基本型,所有有色稻都是由具有这种基本型酶谱带的色稻演化而成。根据分析和推论,共制作出5个分子进化树,进化步骤最少的为8,最多为12。
This paper reports the preliminary results of the molecular evolution of Chinese special rice (paddy rice). In this study, non-colored rice varieties distributed in different provinces of China were used to study the enzyme bands of esterase isozymes and peroxidase isozymes as molecular markers. Based on the basic principles of branch taxonomy, PAUP2.4 was prepared. 1, PAUP30 and Hennig86 three computer software packages, manual analysis of esterase isozyme bands and molecular phylogenetic tree production. The results showed that the types 1, 4 and 7 of esterase isozymes are the basic types, and all the colored rice are evolved from the rice with this basic type of zymogram. Based on analysis and inference, a total of five molecular phylogenetic trees were produced, with a minimum of eight evolutionary steps and a maximum of 12.