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为获取有效的日本血吸虫病早期诊断靶抗原分子。以感染前和感染后2周、4周、6周兔混合血清以及急性、慢性日本血吸虫病人和正常人血清,用Westernblot对日本血吸虫成虫可溶性抗原(AWA)和虫卵可溶性抗原(SEA)进行了全面的分析与筛选。SEA140kDa和AWA54kDa、21kDa、20kDa分子出现最早,能被感染后2周兔血清所识别;SEA69kDa、50kDa、45kDa、38kDa和AWA72kDa、45kDa、34kDa、15kDa相继能被感染后4周兔血清所识别;其中SEA140kDa、50kDa、45kDa、38kDa和AWA34kDa、21kDa、54kDa、15kDa与病人血清反应同6周兔血清反应效果相仿,均为免疫反应主带,且在SDS-PAGE上有对应的蛋白主带,具有潜在的早期诊断价值。进一步对SEA进行抗体类型的分析,发现SEA能刺激机体产生IgG、IgM和IgA反应,其中SEA50kDa、45kDa、38kDa三个抗原分子均能被IgG、IgM、IgA三种抗体所识别,且均为反应主带;SEA140kDa以IgM反应带最深;SEA100kDa反应带仅出现于病人血清,以IgM反应最强,且其与IgM反应带在急性病人血清明显深于慢性病人血清,表明针对IgM的SEA100kDa分子也具有一定的早期诊断和区分病程的价值。SEA140kDa、50kDa、45kDa、38kDa和AWA34kDa、21kDa、54kDa、15kDa和针对IgM的SEA100kDa分子是具有潜在早期诊断价值的优势靶抗原分子。
To obtain effective schistosomiasis japonica early diagnosis of target antigen molecules. Serums of adult worms soluble antigen (AWA) and soluble egg antigen (SEA) of Schistosoma japonicum were detected by western blot using mixed serum of rabbits before and 2 weeks, 4 weeks and 6 weeks after infection as well as acute and chronic Schistosoma japonicum and normal human serum Comprehensive analysis and screening. The SEA140kDa and AWA54kDa, 21kDa and 20kDa molecules appeared earliest and could be recognized by rabbit serum after 2 weeks of infection. SEA69kDa, 50kDa, 45kDa, 38kDa and AWA72kDa, 45kDa, 34kDa and 15kDa were recognized by rabbit sera 4 weeks after infection; SEA140kDa, 50kDa, 45kDa, 38kDa and AWA34kDa, 21kDa, 54kDa and 15kDa have the same effect as the serum reaction of the 6-week-old rabbits, all of them are the main immunoreactive bands and have the corresponding protein main band on SDS-PAGE. The early diagnosis value. Further analysis of the antibody types of SEA revealed that SEA can stimulate the body to produce IgG, IgM and IgA reactions, of which SEA50kDa, 45kDa, 38kDa three antigen molecules can be IgG, IgM, IgA three antibody recognition, and are reaction SEA140kDa with the deepest IgM reaction zone; SEA100kDa reaction zone only appeared in the patient’s serum, the strongest IgM response, and its reaction with the IgM in the acute patient serum was significantly deeper than the serum of patients with chronic, that SEA100kDa molecules against IgM also has Certain early diagnosis and differentiation of disease value. The SEA 140 kDa, 50 kDa, 45 kDa, 38 kDa and AWA 34 kDa, 21 kDa, 54 kDa, 15 kDa and SEA 100 kDa molecules against IgM are the predominant target antigen molecules with potential early diagnostic value.