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实验采用细胞外玻璃微电极采集豚鼠海马神经元放电信号,并将信号转化为峰峰间期(interspike interval,ISI)以研究麻醉和清醒状态海马锥体细胞自发放电线性和非线性特点。实验建立了豚鼠海马锥体细胞与中间神经元电生理鉴别标准;麻醉和清醒状态下豚鼠海马CAl和CA3区锥体细胞自发放电频率、时程、复杂度等无显著区别;麻醉组豚鼠海马锥体细胞ISI序列的复杂度小于清醒组,锥体细胞分型和ISI变异度等表现不同。实验表明,麻醉和清醒状态下豚鼠海马锥体细胞自发放电呈不同线性和非线性特征。传统和非线性研究手段的结合,可能较全面地反映海马锥体细胞自发放电特性。
The extracellular glass microelectrode was used to collect the discharge signal of hippocampal neurons in guinea pig and the signal was converted into interspike interval (ISI) to study the linear and nonlinear characteristics of spontaneous discharge of hippocampal pyramidal cells in anesthetized and awake states. The electrophysiological criteria of pyramidal cells and interneurons in guinea pig hippocampus were established experimentally. There was no significant difference in spontaneous discharge frequency, duration and complexity of hippocampal CA1 and CA3 pyramidal cells in anesthetized and awake guinea pigs. The complexity of somatic ISI sequence is less than that of awake group, pyramidal cell type and ISI variation. Experiments show that under anesthetized and awake guinea pig hippocampal pyramidal cells spontaneous discharge showed different linear and nonlinear characteristics. The combination of traditional and non-linear research methods may reflect the spontaneous discharge characteristics of pyramidal cells in the hippocampus.