论文部分内容阅读
根据p21基因序列设计合成特异性检测p21mRNA的分子信标,发展了一种体外快速、定量检测p21mRNA的新方法,将其用于肿瘤细胞p21mRNA表达水平的检测.结果发现:抑制p53表达引起CNE2细胞中p21mRNA表达水平降低,转染ING1诱导MCF-7细胞中p21mRNA表达上调,5-氟尿嘧啶处理MCF-7细胞后p21mRNA表达水平呈浓度和时间相关性变化.这种特异性强、操作快速、简便的新方法有望广泛用于各类样品中p21mRNA表达水平检测.
According to the sequence of p21 gene, we designed and synthesized the specific molecular beacon of p21mRNA, and developed a new method for rapid and quantitative detection of p21mRNA in vitro.It was used to detect the expression of p21mRNA in tumor cells.It was found that the inhibition of p53 expression caused CNE2 cells P21mRNA in MCF-7 cells transfected with ING1 was upregulated, and p21mRNA expression level in MCF-7cells treated with 5-Fluorouracil showed a concentration-dependent and time-dependent change.This specificity was high and the operation was quick and easy The new method is expected to be widely used for the detection of p21 mRNA expression in various samples.