作者介绍了一种根据多聚酶链反应而发展的检测血清性丙型肝炎病毒(HCV)RNA的敏感方法。为了选择高效并有特异性的引物,从日本7例慢性非甲非乙型肝炎患者的肝脏标本中分离出大小为282碱基对(bp)的HCV cDNA克隆,并将其与Chiron克隆的顺序进行比较。其核苷酸顺序与Chiron HCV有76～77%的同源性。有两个引物碱基对的顺序有高度的保守性。引物JK3779(5′-GAGTGCG…3′)起始于Chiron HCV基因组图的第3779位;引物JK3966 R在第3966位 The authors describe a sensitive method for detecting serological hepatitis C virus (HCV) RNA that has been developed on the basis of polymerase chain reaction. In order to select efficient and specific primers, HCV cDNA clones of 282 base pairs (bp) were isolated from liver samples of 7 chronic non-A, non-B hepatitis patients in Japan and compared with Chiron cloning sequence Compare. Its nucleotide sequence is 76-77% homologous to Chiron HCV. The sequence of two primer base pairs is highly conserved. Primer JK3779 (5’-GAGTGCG ... 3 ’) starts at position 3779 of the Chiron HCV genome map; primer JK3966R at position 3966