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目的:探讨常压不同低氧(14.5%、12.6%氧含量)环境下(相当于模拟海拔3000m和4000m)高住低练对大鼠心肌线粒体活性氧(ROS)的影响,为运动与低氧适应的应用研究提供参考。方法:120只SD大鼠,随机分为12组,分别为低住安静组、低住低练组、急性高住安静组、急性高住运动组、急性高住低练组、高住安静组和高住低练组,5个高住组又分为常压14.5%氧含量和12.6%含氧量2个亚组。采用二氯荧光素双醋酸盐(DCFH-DA)荧光探针观察线粒体ROS的变化。结果:急性低氧应激后,大鼠心肌线粒体ROS呈上升趋势。与低住安静组相比,模拟海拔3000m和4000m急性高住安静组和急性高住运动组线粒体ROS均显著升高(P<0.05,P<0.01);与低住低练组相比,急性高住运动组均显著升高(P<0.01),模拟海拔4000m高住低练组显著升高(P<0.05);与急性高住运动组相比,模拟海拔3000m急性高住低练组和高住低练组均显著下降(P<0.05),可见模拟海拔3000m高住低练能使ROS生成减少。结果提示:心肌线粒体ROS可能参与低氧适应的形成和发展,高住低练有利于缺氧耐受性的形成。
OBJECTIVE: To investigate the effects of high-living-low training at high atmospheric pressure and low oxygen (14.5%, 12.6% oxygen) on myocardial mitochondrial reactive oxygen species (ROS) in myocardium under exercise and hypoxia Adaptation of applied research to provide a reference. Methods: One hundred and twenty SD rats were randomly divided into 12 groups: low-lying and quiet group, low-lying low-training group, acute high-living-quiet group, acute high-activity group, And high living low training group, five high-living group is divided into 14.5% atmospheric pressure and 12.6% oxygen content of 2 subgroups. The change of mitochondrial ROS was observed by fluorescent probe of dichlorofluorescein diacetate (DCFH-DA). Results: After acute hypoxia stress, ROS in mitochondria of rats increased. Compared with the low-lying and quiet group, mitochondrial ROS were significantly increased (P <0.05, P <0.01) in acute high-living-quiet group and acute high-activity group at simulated altitude of 3000m and 4000m, (P <0.01). Compared with the acute high-exercise group, the simulated high-altitude low-altitude training group with an altitude of 3000m and the high-altitude low-altitude training group High-living low training group were significantly decreased (P <0.05), we can see the simulation altitude 3000m high live low training can reduce ROS generation. The results suggest that myocardial mitochondrial ROS may be involved in the formation and development of hypoxia adaptation, high living and low training is conducive to the formation of hypoxic tolerance.