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目的建立稳定表达荧光素酶报告基因的人肺癌细胞系,并将该肺癌细胞接种于严重联合免疫缺陷(SCID)小鼠的皮下,构建异种移植动物模型,为下一步进行肺癌进展、转移以及药物敏感性等相关的分子影像学研究奠定基础。材料与方法利用表达重组荧光素酶基因的慢病毒载体(Lenti-Luc)感染人肺癌细胞系NCI-H460,获得稳定表达荧光素酶基因的肺癌细胞,将该细胞接种于SCID小鼠的皮下。肿瘤细胞接种2周后,利用生物发光成像系统及MRI对肿瘤进行检测,确定肿瘤的大小和位置,并进行组织病理学检查验证。结果接种肺癌细胞的4只SCID小鼠中,均生长出肿瘤。生物发光检测和MRI的结果相符,病理证实小鼠皮下肿块为癌组织。结论成功建立了稳定表达荧光素酶报告基因的肺癌细胞系及异种移植动物模型。荧光素酶活体发光是一种进行肿瘤分子影像学研究的重要手段。
Objective To establish a human lung cancer cell line stably expressing a luciferase reporter gene and inoculate the lung cancer cell subcutaneously in severe combined immunodeficiency (SCID) mice to construct a xenotransplantation animal model for the next step in lung cancer progression, metastasis, and drugs Sensitivity and other related molecular imaging studies lay the foundation. Materials and Methods Human lung cancer cell line NCI-H460 was infected with Lenti-Luc expressing a recombinant luciferase gene, and a lung cancer cell stably expressing the luciferase gene was obtained. The cells were seeded subcutaneously in SCID mice. Two weeks after the tumor cells were inoculated, tumors were detected using a bioluminescent imaging system and MRI to determine the size and location of the tumors, and histopathological examinations were performed. As a result, tumors grew in all 4 SCID mice inoculated with lung cancer cells. The results of bioluminescence detection and MRI were consistent with pathological findings. The subcutaneous mass in mice was a cancerous tissue. Conclusion The lung cancer cell line and xenograft animal model that stably expressed luciferase reporter gene were successfully established. Luciferase living luminescence is an important method for tumor molecular imaging studies.