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用采自云南昆明、曲靖和玉溪等地的8份金盏花种质资源为试材,利用改良CTAB法提取DNA,筛选引物,优化PCR反应体系,对其进行遗传多样性RAPD(random amplified polymorphic DNA)和NTSYS2聚类分析,以建立金盏花亲缘关系分析方法,明确分布在云南省各地的金盏花种质资源之间的亲缘关系和遗传多样性。结果表明:优化的PCR反应体系能达到良好的扩增效果,30条RAPD引物在8份金盏花种质资源中共产生165个位点,其中45个位点具有遗传多态性,约占27.28%。CK与7号的相似性最小,用CK和7号作为亲本的后代会有较大的分离。试验表明金盏花种植资源遗传多样性丰富,遗传关系与其地理关系密切相关,但并非严格的限聚在一起,需结合表型更深入地分析。
Using eight Calendula officinalis germplasm resources collected from Kunming, Qujing and Yuxi in Yunnan as materials, the improved CTAB method was used to extract DNA, select the primers and optimize the PCR reaction system. The genetic diversity RAPD (random amplified polymorphic DNA) and NTSYS2 cluster analysis to establish the genetic relationship analysis method of Calendula officinalis, to clarify the genetic relationship and genetic diversity among the germplasm resources of Calendula officinalis distributed in Yunnan Province. The results showed that the optimized PCR reaction system could achieve good amplification effect. Twenty-five RAPD primers generated 165 loci in 8 accessions of Calendula officinalis, of which 45 loci had genetic polymorphism, accounting for 27.28 %. CK and 7 the smallest similarity, with CK and 7 as the progeny of offspring will have a greater separation. Experiments show that planting resources of marigolds rich in genetic diversity, genetic relationship and its geographical relationship is closely related, but not strictly limited together, need to be combined with the phenotype more in-depth analysis.