单纯疱疹病毒性脑炎(HSE)早期确诊困难。我们用ELISA单克隆抗体双夹心法测脑脊液(CSF)中单纯疱疹病毒(HSV)抗原,并对部分标本测定HSV-Ⅰ和HSV-Ⅱ型抗体。 材料和方法 一、研究对象:14例病员,男女各7例,年龄平均34.18岁(17～49岁)。全部病例均作脑电图、头颅CT或MRI、CSF等检查。病毒性脑炎5例,散发性脑炎8例,多颅神经炎1例。 二、方法:病人入院48h内即抽取腰池CSF2～3ml和静脉血3ml并分离血清(S)送检。 Herpes simplex encephalitis (HSE) early diagnosis difficult. We measured the herpes simplex virus (HSV) antigen in cerebrospinal fluid (CSF) using double-sandwich ELISA with monoclonal antibodies against HSV-I and HSV-II in some samples. Materials and Methods First, the research object: 14 patients, 7 males and 7 females, mean age 34.18 years (17 to 49 years old). All cases were EEG, cranial CT or MRI, CSF and other tests. 5 cases of viral encephalitis, sporadic encephalitis in 8 cases, 1 case of cranial neuritis. Second, the method: Within 48h after admission, patients were drawn waist CSF 2 ~ 3ml and venous blood 3ml and sera (S) for submission.