在大鼠角朊细胞原代培养的基础上 ,建立导电聚吡咯膜角朊细胞原代培养技术 ,并探讨了导电聚吡咯膜对角朊细胞体外生长作用的影响。实验结果显示 ,单纯聚吡咯膜或导电刺激对角朊细胞生长的作用和对照组相比没有显著性差异 ,而导电聚吡咯膜可以有效地促进角朊细胞的生长和增殖 ,且对不同种植密度、不同培养时间的大鼠角朊细胞的促生长作用也有显著性差别。在降低常规培养条件下所需要的细胞种植密度的同时 ,可以将角朊细胞生长融合培养时间缩短约 2天 Based on the primary culture of rat keratinocytes, primary culture of keratinocytes with conductive polypyrrole was established, and the effect of conductive polypyrrole on the growth of keratinocytes in vitro was also discussed. The experimental results showed that the effect of pure polypyrrole film or conductive stimuli on keratinocyte growth was not significantly different from the control group, while the conductive polypyrrole film can effectively promote the growth and proliferation of keratinocytes, and the different planting density , Different culture time rat keratinocyte growth promoting effect also have significant difference. While reducing the cell density required for conventional culture, keratinocyte growth can be shortened approximately 2 days