论文部分内容阅读
该实验构建了慢病毒介导(lentivirus,LV)的miR-4258-siRNA,探讨干扰miR-4258对肾小管上皮细胞(HK-2)转分化的影响。将HK-2转分化细胞分为正常组、阴性对照组和干扰组;荧光定量PCR方法检测miR-4258的表达情况;免疫印迹检测Fibronectin、E-cadherin和α-SMA的表达情况。结果显示,miR-4258-siRNA-LV可显著降低miR-4258的表达水平,干扰组的Fibronectin和α-SMA的相对表达量较阴性对照组显著下调;而E-cadherin的相对表达量较阴性对照组明显上调。研究说明,通过慢病毒介导的miR-4258-siRNA-LV下调miR-4258的表达,可抑制波动高糖诱导的HK-2细胞转分化过程。
This experiment constructed lentivirus-mediated miR-4258-siRNA to investigate the effect of miR-4258 on the transdifferentiation of renal tubular epithelial cells (HK-2). The transfected HK-2 cells were divided into normal group, negative control group and interference group. The expression of miR-4258 was detected by real-time PCR. The expression of Fibronectin, E-cadherin and α-SMA were detected by Western blotting. The results showed that miR-4258-siRNA-LV significantly reduced the expression of miR-4258, and the relative expression of Fibronectin and α-SMA in the interference group was significantly lower than that in the negative control group; while the relative expression of E-cadherin was lower than that in the negative control Group was significantly increased. The research shows that miR-4258-siRNA-LV can down-regulate the expression of miR-4258 by lentivirus and can inhibit the transdifferentiation of HK-2 cells induced by high glucose.