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目的探讨内皮型一氧化氮合酶(eNOS)基因及内皮素受体A型(EDNRA)基因与颅内动脉瘤性蛛网膜下腔出血(aSAH)的预后的相关性。方法 aSAH共60例,详细收集所有患者的一般情况,预后标准采用改良Rankin量表(modified Rankin Scale,mRS)进行评分。应用限制性片段长度多态性聚合酶链反应(PCR-RFLP)方法对EDNRA基因3个多态位点(rs5335、rs6842241、rs6841581位点)及eNOS基因2个多态位点(G-894T、T-786C位点)进行研究,分析上述多态位点基因型及等位基因频率在不同预后病例中的分布。结果预后良好38例(63.3%),预后差者22例(36.7%)。预后良好组与预后差组EDNRA与eNOS基因型及等位基因频率分布差异无统计学意义。结论在本组病例中,eNOS及EDNRA基因型及等位基因分布与aSAH的预后无明显相关。
Objective To investigate the relationship between the expression of endothelial nitric oxide synthase (eNOS) gene and endothelin receptor type A (EDNRA) gene and the prognosis of intracranial aneurysmal subarachnoid hemorrhage (aSAH). Methods A total of 60 patients with aSAH were enrolled in this study. The general condition of all patients was collected. The prognostic criteria were scored using modified Rankin Scale (mRS). Three polymorphic sites of EDNRA gene (rs5335, rs6842241, rs6841581) and two polymorphisms of eNOS gene (G-894T, T-786C locus) to study the distribution of genotype and allele frequencies of the above polymorphic loci in different prognostic cases. Results The prognosis was good in 38 cases (63.3%), and the prognosis was poor in 22 cases (36.7%). There was no significant difference in genotype and allele frequencies of EDNRA and eNOS between the good prognosis group and the poor prognosis group. Conclusion In this group of patients, eNOS and EDNRA genotype and allele distribution and prognosis of aSAH no significant correlation.